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Detached leaflets or tuber slices were inoculated with a 30 μl droplet of sporangia/zoospore suspension (50 sporangia/μl).
After incubation of 6 days at 16°C, in high humidity and, in case of leaflets, under constant light of about 1,600 lx, they were scored using a 1-9 scale, where 9 is maximum resistant .
The flower colours of the parents and examples from the progeny are shown in Figure polymerase (0.05 U/μl) and 10-30 ng of template DNA.
The PCR program was 94°C - 180 s; 40 cycles of: 94°C - 30 s, 55°C - 45 s, 72°C - 90 s; 72°C - 420 s; where the annealing temperature was modified depending on primers used, this is indicated in Additional file The normality of the distribution of the phenotypic data was checked by the Kolmogorov-Smirnov test.
The detached leaflet tests were replicated as follows: 3 leaflets/genotype × 2 replications × 2 dates were tested in 2007, 3 leaflets/genotype × 2 replications in 2008 and 3 leaflets/genotype × 2 replications × 2 dates in 2010.
Three tuber slices/genotype × 2 replications were tested in 2006, 20. possessing the R gene, when its mean resistance score in both detached leaflet and in tuber slice tests was ≥ 7.
Cvs Gloria and Robijn and clone DG 94-668 proved more susceptible than expected.
It was linked, at a distance of 3.4 c M, to violet flower colour most likely controlled by the previously described locus.
) was conducted at Southeast Poland in Boguchwala, where the weather conditions were favorable for severe late blight infection.
Material was planted in randomized 4-hill plots, in two replications in 2008 and in one replication in 2009.
However, to obtain durable resistance in potato cultivars more genes are needed to be identified to realize strategies such as gene pyramiding or use of genotype mixtures based on diverse genes.
was mapped to potato chromosome X using Diversity Array Technology (DAr T) and sequence-specific PCR markers.